Developmental changes in zebrafish embryos exposed to bisphenol A leeched from commonly used plastics

Bisphenol A, an estrogen mimicker, is found in the commonly used plastic, polycarbonate. Zebrafish embryos were raised in water that had been heated in polycarbonate water bottles by either microwaving for 5 minutes, or boiling for one hour. Near total mortality was seen in 7 days for the embryos growing in bottle-microwaved water, and 9 days for those raised in bottle-boiled water. Zebrafish embryos were also grown in the following known concentrations of BPA: 2mg/l, 14mg/l, 16mg/l, 50mg/l, 135mg/l, and 235mg/l. A stepwise relationship between concentration of BPA and embryo lifespan was seen, and the lowest observed adverse effect level was at 14mg/l. Developmental changes in zebrafish 4